A queen cup according to the present invention comprises: a receiving part having an inlet formed at one side thereof and formed as a container body for receiving royal jelly or eggs; and a support configured to surround and support the receiving part so that the inlet is opened, wherein, when closely contacting a neighboring support, the outer surface of the support has a shape that is entirely in close contact with the outer surface of the neighboring support so that no empty space is formed between the closely contacted supports. The breeding frame using the queen cup according to the present invention comprises a support plate on which queen cups are arranged in at least one direction to have the outer surfaces of neighboring queen cups to closely contact each other so that no empty space is formed between the outer contact surfaces of the neighboring queen cups, and which supports the queen cups while being fixed to or combined with the opposite side of the inlet of the arranged queen cups. The breeding
