CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE;UNIVERSITE CLAUDE BERNARD LYON 1
发明人:
COMPAGNON ISABLLE,SCHINDLER BAPTISTE
申请号：
FR20160053425
公开号：
FR3050271(B1)
申请日:
2016.04.18
申请国别(地区):
法国
年份:
2018
代理人:
摘要：
The present application describes a method for sequencing oligosaccharides, which enables the primary sequence of an oligosaccharide with unknown structure to be identified, including the composition of same in monosaccharides, the position (regiochemistry) and configuration (stereochemistry) of the glycosidic bonds, the nature and position of the functional modifications, and the branched structure thereof, including in particular identifying the reductive end. Said method of sequencing oligosaccharides comprises the steps of i. fragmentation of the oligosaccharides into disaccharides and monosaccharides while preserving the molecular structure of the constituents as present in the oligosaccharide to be sequenced, ii. separating each previously obtained disaccharide and monosaccharide by mass spectrometry, iii. analysing, by vibrational IR (infrared) spectroscopy, each previously separated disaccharide and monosaccharide, iv. identifying the structure of each disaccharide and monosaccharide by comparing the
