PROBLEM TO BE SOLVED: To propose an evaluation method of a medium for mushroom cultivation that can easily and accurately evaluate whether biomass to be used as a medium for mushroom cultivation is suitable as a medium.SOLUTION: The evaluation method of the medium for mushroom cultivation analyzes unused state (unused medium), used state (waste medium), and the state of being reproduced by software hydrothermal treatment (reproduction medium) with regard to the biomass to be used as the medium for mushroom cultivation. In their respective medium, a specific marker, which is a unique component, is used as an indicator of the medium for evaluation. The quality of the mushroom culture medium can be evaluated without culture.SELECTED DRAWING: Figure 3-5COPYRIGHT: (C)2016,JPO&INPIT【課題】きのこ栽培用の培地として用いるバイオマスが培地として適しているか否かを、簡単かつ精度良く評価できるきのこ栽培用培地の評価方法を提案すること。【解決手段】きのこ栽培用培地の評価方法は、きのこ栽培用の培地として用いるバイオマスについて、未使用の状態(未使用培地)、使用済みの状態(廃培地)、ソフト水熱処理によって再生された状態(再生培地)を分析し、それぞれの培地に固有の成分である特定マーカーを、培地評価用の指標として用いる。培養を経ずに、きのこ培地の品質を評価できる。【選択図】図3-5