ШЕЕР Джастин (US),ШПИСС Кристоф (US),ЯНСУРА Дэниел Дж. (US)
申请号：
RU2012149858/10
公开号：
RU2012149858A
申请日:
2011.04.22
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A method for producing a heteromultimeric protein comprising a first polypeptide containing a hinge region and having a first heterodimerization domain, and a second polypeptide containing a hinge region and having a second heterodimerization domain, wherein said second heterodimerization domain interacts with said first heterodimerization domain, and where the first and second polypeptides containing a hinge region are linked to each other by at least one interchain disulfide bond； wherein said method comprises the steps of: (a) preparing a purified first polypeptide containing a hinge region and having a first heterodimerization domain； (b) preparing a purified second polypeptide containing a hinge region and having a second heterodimerization domain； (c) combining the first and second polypeptides containing hinge region； (d) refolding the first polypeptide containing the hinge region with the second polypeptide containing the hinge region, under conditions conducive to multimerization of the selected polypeptides ide； and (e) isolating the heteromultimeric protein complex. 2. A method for producing a heteromultimeric protein comprising a first polypeptide containing a hinge region and having a first heterodimerization domain, and a second polypeptide containing a hinge region and having a second heterodimerization domain, wherein said second heterodimerization domain interacts with said first heterodimerization domain, and where the first and second polypeptides containing a hinge region are connected to each other by at least one interchain disulfide bond； moreover, this method includes1. Способ получения гетеромультимерного белка, включающего первый полипептид, содержащий шарнирную область и имеющий первый домен гетеродимеризации, и второй полипептид, содержащий шарнирную область и имеющий второй домен гетеродимеризации, где указанный второй домен гетеродимеризации взаимодействует с указанным первым доменом гетеродимеризации, и где первый и второй полипепти
