An omega-3 oil concentrate comprising at least 70 weight percent of eicosapentaenoic acid ["EPA"； cis-5, 8, 11, 14, 17-eicosapentaenoic acid； omega-3], measured as a weight percent of oil, and substantially free of docosahexaenoic acid, said concentrate obtained from a microbial oil having 30 to 70 weight percent of eicosapentaenoic acid, measured as a weight percent of total fatty acids, and substantially free of docosahexaenoic acid and wherein said microbial oil is obtained from a microorganism that accumulates in excess of 25% of its dry cell weight as oil. Also disclosed are methods of making such eicosapentaenoic acid concentrates.
