FISCHER, STEPHAN,HESSE, FRIEDERIKE,KNOETGEN, HENDRIK,LANG, KURT,METZGER, FRIEDRICH,REGULA, JOERG THOMAS,SCHANTZ, CHRISTIAN,SCHAUBMAR, ANDREAS,SCHOENFELD, HANS JOACHIM
申请号：
CA2662062
公开号：
CA2662062C
申请日:
2007.08.29
申请国别(地区):
CA
年份:
2015
代理人:
摘要：
Method for the production of a lysine-PEGylated IGF-I or IGF-I variant, said variant comprising one or two amino acid(s) selected from the group consisting of lysine 27, 65 and/or 68 substituted independently by another polar amino acid, characterized in cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding a fusion protein comprising said IGF-I or IGF-I variant N-terminally linked to the C-terminus of a propeptide, that said propeptide ends C-terminally with amino acids -Y-Pro, wherein Y is selected from the group consisting of Pro, Pro-Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr-Pro, Arg-Pro, or Pro-Arg-Pro, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering said PEGylated IGF-I or IGF-I variant. The PEGylated IGF-I or IGF-I variant is useful for the treatment of neurodegenerative disorders like Alzheimer's Disease.
