Schaubmar, Andreas,Hesse, Friederike,Schoenfeld, Hans Joachim,Regula, Joerg Thomas,Knoetgen, Hendrik,Lang, Kurt,Fischer, Stephan,Metzger, Friedrich,Schantz, Christian
申请号：
AU2007291502
公开号：
AU2007291502B2
申请日:
2007.08.29
申请国别(地区):
AU
年份:
2012
代理人:
摘要：
Method for the production of a lysine-PEGylated IGF-I or IGF-I variant, said variantcomprising one or two amino acid(s) selected from the group consisting of lysine27, 65 and/or 68 substituted independently by another polar amino acid, characterizedin cultivating a prokaryotic host cell comprising an expression vector containinga nucleic acid encoding a fusion protein comprising said IGF-I or IGF-I variantN-terminally linked to the C-terminus of a propeptide, that said propeptideends C-terminally with amino acids -Y-Pro, wherein Y is selected from the groupconsisting of Pro, Pro-Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr-Pro, Arg-Pro,or Pro-Arg-Pro, recovering and PEGylating said fusion protein, cleaving saidPEGylated fusion protein with IgA protease, and recovering said PEGylated IGF-Ior IGF-I variant. The PEGylated IGF-I or IGF-I variant is useful for the treatmentof neurodegenerative disorders like Alzheimers Disease.
