Stephan Fischer,Friederike Hesse,Hendrik Knoetgen,Kurt Lang,Joerg Thomas Regula,Christian Schantz,Andreas Schaubmar
申请号：
US12438154
公开号：
US08552158B2
申请日:
2007.08.29
申请国别(地区):
US
年份:
2013
代理人:
摘要：
Method for the production of IGF-I, characterized by cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding a fusion protein comprising said IGF-I N-terminally linked to the C-terminus of a propeptide, whereby said propeptide ends C-terminally with amino acids -Y-Pro, wherein Y is selected from the group consisting of Pro, Pro-Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr-Pro, Arg-Pro, or Pro-Arg-Pro, recovering and cleaving said fusion protein with IgA protease, and recovering said IGF-I. IGF-I is useful for the treatment of neurodegenerative disorders like Alzheimers Disease.
