Schaubmar, Andreas,Knoetgen, Hendrik,Fischer, Stephan,Hesse, Friederike,Lang, Kurt,Schantz, Christian,Regula, Joerg Thomas
申请号：
AU2007291501
公开号：
AU2007291501B2
申请日:
2007.08.29
申请国别(地区):
AU
年份:
2012
代理人:
摘要：
Method for the production of IGF-I, characterized by cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding a fusion protein comprising said IGF-I N-terminally linked to the C-terminus of a propeptide, whereby said propeptide ends C-terminally with amino acids -Y-Pro, wherein Y is selected from the group consisting of Pro, Pro-Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr-Pro, Arg-Pro, or Pro-Arg-Pro, recovering and cleaving said fusion protein with IgA protease, and recovering said IGF-I. IGF-I is useful for the treatment of neurodegenerative disorders like Alzheimer's Disease.
