БЕНАТУИЛ Лоренцо (US),ГХАЮР Тарик (US),ГУДРО Кэрри Л. (US),ИСАКСОН Питер С. (US),ЗАЛЬФЕЛД Йохен (US)
申请号：
RU2012147249/10
公开号：
RU2012147249A
申请日:
2011.04.06
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A humanized binding protein containing an antigen binding domain capable of binding human tumor necrosis factor alpha (TNF-α), wherein the antigen binding domain contains at least one CDR containing the amino acid sequence of residues 31-35 of SEQ ID NO: 22； residues 50-65 of SEQ ID NO: 22； residues 98-106 of SEQ ID NO: 22； residues 24-34 of SEQ ID NO: 23； residues 50-56 of SEQ ID NO: 23； or residues 89-97 of SEQ ID NO: 23, wherein the binding protein contains a human acceptor framework. 2. A binding protein according to claim 1, wherein the binding protein contains at least 3 CDRs. The binding protein according to claim 2, where at least 3 CDRs contain a variable domain CDR kit containing (a) residues 31-35 of SEQ ID NO: 22； residues 50-65 of SEQ ID NO: 22 and residues 98-106 of SEQ ID NO: 22； and (b) residues 24-34 of SEQ ID NO: 23； residues 50-56 of SEQ ID NO: 23 and residues 89-97 of SEQ ID NO: 23.4. The binding protein of claim 3, wherein the antigen binding domain comprises an amino acid sequence containing residues 31-35 of SEQ ID NO: 22； residues 50-65 of SEQ ID NO: 22； residues 98-106 SEQ ID NO: 22； residues 24-34 of SEQ ID NO: 23； residues 50-56 of SEQ ID NO: 23 and residues 89-97 of SEQ ID NO: 23.5. The binding protein of claim 1, wherein the antigen binding protein comprises a VH.6 region. The binding protein of claim 5, wherein the VH region contains the amino acid sequence of SEQ ID NO: 24, 25, 28, 29, 30, 31, 32, or 33.7. The binding protein of claim 1, wherein the antigen binding domain comprises a VL.8 region. The binding protein of claim 7, wherein the amino acid sequence of the VL region is SEQ ID NO: 26, 27, 34, 35, or 36.9. The binding protein of claim 7, wherein the antigen binding domain comprises a VH region and a VL region. The binding protein of claim 9, wherein the VH region contains the amino acid sequence of SEQ ID NO: 24, 25, 28, 29, 30, 31, 32 or 33, and the VL region contains the amino acid sequence of SEQ ID NO: 26, 27, 34, 35 or
