ポリケタイド合成酵素遺伝子をレポーター遺伝子として利用する方法
- 专利权人:
- 发明人:
- 申请号:
- JP20100058032
- 公开号:
- JP5757380(B2)
- 申请日:
- 2010.03.15
- 申请国别(地区):
- 日本
- 年份:
- 2015
- 代理人:
- 摘要:
PROBLEM TO BE SOLVED: To provide a new reporter gene which can be used in fungi, and is excellent in detection sensitivity.
SOLUTION: Using a fungus which has a polyketide synthase gene as a reporter gene, the expression of the gene is determined by Flaviolin produced in a medium as an indicator. A nucleic acid structure includes a transcription regulatory region with its functional known or unknown, and a polyketide synthase gene expressed under the control of the transcription regulatory region, wherein the transcription regulatory region includes one promoter region selected from the group consisting of a promoter induced by an agent for improving the plant disease resistance, a promoter induced by electron transfer system inhibitor, and a promoter induced by ergosterol biosynthesis inhibitor.
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- 来源网站:
- 中国工程科技知识中心