A low toxicity sophorolipid-containing composition is provided. When the total amount of the acid type sophorolipid, the lactone type sophorolipid, and the fatty acid and the hydroxy fatty acid is 100% by mass, the coloring component derived from the SL-producing yeast culture, the acid type sophorolipid, the fatty acid and the hydroxy fatty acid are contained at least. A low-toxic sophorolipid-containing composition characterized by the following ratios in terms of dry weight: (1) acid sophorolipid: 94 to 99.99% by mass, (2) lactone sophorolipid: 0 to 2 mass %, (3) Total amount of fatty acid and hydroxy fatty acid: 0.01 to 4% by mass.低毒性ソホロリピッド含有組成物を提供する。ＳＬ産生酵母培養物に由来する着色成分、酸型ソホロリピッド、脂肪酸及びヒドロキシ脂肪酸を少なくとも含有し、酸型ソホロリピッド、ラクトン型ソホロリピッド、並びに脂肪酸及びヒドロキシ脂肪酸の総量を１００質量％とした場合に、それぞれの割合が乾燥重量に換算して下記であることを特徴とする、低毒性ソホロリピッド含有組成物；（1）酸型ソホロリピッド：９４～９９．９９質量％、（2）ラクトン型ソホロリピッド：０～２質量％、（3）脂肪酸及びヒドロキシ脂肪酸の総量：０．０１～４質量％。
