The problem of the present invention is to provide a herbicidal composition that does not permit easy generation of fatty acid odor, shows low toxicity of decomposed material, is superior in the long-term stability, permits wide selectivity in obtaining the starting material, and is suitable for the environment. As a solving means of the problem, the present invention provides a herbicidal composition containing, as an active ingredient, glycerol fatty acid ester obtained from fatty acid having an even-numbered carbon number of 6 - 12 and glycerol.