GILLJAM, Gustav,JERNBERG, Mats,WINGE, Stefan,NEISSER-SVAE, Andrea
申请号：
ES08787446
公开号：
ES2572352T3
申请日:
2008.08.25
申请国别(地区):
ES
年份:
2016
代理人:
摘要：
A method of isolation and purification of a target protein by chromatography, in which chromatography eliminates or decreases the amount of prions (PrPSC), which comprises the steps of: - contacting a potentially contaminated sample with PrPSC comprising a protein target, with a multimodal chromatographic material, in which the multimodal chromatographic material contains a negatively charged 2- (benzoylamino) butanoic acid ligand； - establish the conditions of the buffer so that the target protein binds to the multimodal chromatographic material, while the PrPSCs do not bind the multimodal chromatographic material, in which the chromatographic conditions comprise the following steps: i) use a loading buffer and equilibrium containing tri-n-butyl phosphate and / or Triton X-100 in a concentration ranging from 0.1 to 10% (w / w)； ii) use a wash buffer without solvent and / or non-ionic detergent； iii) use a second wash buffer containing ethylene glycol and / or lysine / arginine ranging from 5 to 30% (w / w) ethylene glycol and 0.2 to 1.5 M lysine / arginine； iv) use a third wash buffer containing sodium chloride in a concentration ranging from 0.5 to 4 M, in particular from 0.5 to 1.5 M； - followed by elution of the target protein.Un procedimiento de aislamiento y purificación de una proteína diana mediante una cromatografía, en el que la cromatografía elimina o disminuye la cantidad de priones (PrPSC), que comprende las etapas de: - poner en contacto una muestra potencialmente contaminada con PrPSC que comprende una proteína diana, con un material cromatográfico multimodal, en el que el material cromatográfico multimodal contiene un ligando de ácido 2-(benzoilamino)butanoico cargado negativamente； - establecer las condiciones del tampón de modo que la proteína diana se una al material cromatográfico multimodal, mientras que las PrPSC no se unan al material cromatográfico multimodal, en el que las condiciones cromatográficas comprenden las siguientes etapas: i) empl
