PROBLEM TO BE SOLVED: To provide a purification method from compositions comprising an antibody and at least one contaminant selected from the group consisting of Chinese Hamster Ovary Proteins (CHOP), leached protein A, DNA, and aggregated CD20 antibodies.SOLUTION: A method comprises, in chromatography, the sequential steps of: (a) loading the composition onto a cation exchange material, in which the pH of the composition is about 4.0-6.0； (b) washing the cation exchange material with a first wash buffer at a pH of about 6.8-9.0； (c) washing the cation exchange material with a second wash buffer at a pH of about 5.0-6.0； and (d) eluting the antibody from the cation exchange material with an elution buffer that has a pH of about 5.0 to 6.0 and conductivity of about 10-100 mS/cm.COPYRIGHT: (C)2015,JPO&INPIT【課題】チャイニーズハムスター卵巣タンパク質（ＣＨＯＰ）、浸出プロテインＡ、ＤＮＡ、および凝集ＣＤ２０抗体からなる群から選択される一以上の汚染物質と抗体とを含有する組成物からの精製方法の提供。【解決手段】クロマトグラフィにおいて、（ａ）陽イオン交換材料に組成物をロードし、ここで組成物は約４．０～約６．０のｐＨであり；（ｂ）約６．８～約９．０のｐＨで第一洗浄ブァッファーを用いて陽イオン交換材料を洗浄し；（ｃ）約５．０～約６．０のｐＨで第二洗浄ブァッファーを用いて陽イオン交換材料を洗浄し；（ｄ）約５．０～約６．０のｐＨ及び約１０ｍＳ／ｃｍ～１００ｍＳ／ｃｍの伝導率を有する溶出ブァッファーを用いて陽イオン交換材料から抗体を溶出させる逐次工程。【選択図】なし
