НОТОН Гейл К. (US),ЗЕЙГЛЕР Фрэнк (US),БАУМГАРТНЕР Марк (US),НИКИ Кайл (US)
申请号：
RU2012104650/15
公开号：
RU2012104650A
申请日:
2010.07.09
申请国别(地区):
RU
年份:
2013
代理人:
摘要：
1. A method of obtaining a composition containing one or more embryonic proteins, comprising culturing cells under hypoxic conditions, in a suitable culture medium on a substrate, thereby obtaining a soluble and insoluble composition containing one or more embryonic proteins. The method of claim 1, wherein the culture medium contains serum. The method of claim 1, wherein the culture medium is serum free. The method of claim 1, wherein the hypoxic oxygen conditions comprise 1-5% oxygen. The method according to claim 1, in which the types of collagen are activated compared to media produced under conditions with an oxygen content of about 15-20%. The method according to claim 5, in which the collagen is selected from collagen type V alpha 1； IX alpha 1； IX alpha 2； VI alpha 2； VIII alpha 1； IV alpha 5； VII alpha 1； XVIII alpha 1 and XII alpha 1.7. The method of claim 1, wherein the Wnt types are activated compared to media produced under conditions with an oxygen content of about 15-20%. The method of claim 7, wherein the Wnt types are wnt 7a and wnt 11.9. The method according to claim 1, in which the types of laminin are activated in comparison with media produced under conditions with an oxygen content of about 15-20%. The method according to claim 9, in which the type of laminin is a laminin 8.11. The method of claim 1, wherein the cell free supernatant is dialyzed, lyophilized and reconstituted in buffer. The method of claim 1, wherein the cell free supernatant is dialyzed, dried, and reconstituted in buffer. The method of claim 1, wherein the cells are fibroblasts. The method of claim 13, wherein the cells are neonatal fibroblasts. The method of claim 1, wherein the cells are stem cells1. Способ получения композиции, содержащей один или более эмбриональных белков, включающийкультивирование клеток в гипоксических условиях, в подходящей культуральной среде на подложке с получением тем самым растворимой и нерастворимой композиции, содержащей один или более эмбрионал
