Systems and methods for establishing growing plant material are presented. The system is configured to grow the plant material in a non-sterile room. One method includes providing a host container comprising sidewalls and a bottom wall that forms an interior processing volume. The method further includes depositing a growth substrate into the interior processing volume and positioning an insert member into the processing volume. The insert member includes a plurality of open-bottom cells, each of the plurality of open-bottom cells includes an open bottom portion and an open top portion. The growth substrate partially extends into each of the plurality of open-bottom cells. The method also includes depositing a plant material composition over the growth substrate. Other systems and methods are presented.
