The present invention relates to a honeybee feed substitute composition using an onion and a preparing method thereof, capable of obtaining honey with more excellent quality. The honeybee feed substitute composition comprises natural sugar derived from onion as an active ingredient, and further comprises at least one of fructose and pollen.본 발명은 꿀벌대체사료에 대한 것으로, 보다 구체적으로는 양파유래 천연당을 포함하는 꿀벌대체사료조성물 및 그 제조방법에 관한 것이다.