An object of the present invention is to provide a seed bottle opening device which can be operated at a low cost, can be easily operated, and can maintain accurate cutting and sterility in an apparatus for providing an opening of a seed bottle set in a fully automatic inoculating machine. Is the subject. An opening device for opening a shoulder portion of a seed germ bottle includes a centering mechanism, a pressing mechanism, and a rotary cutting portion on a main body frame, wherein the centering mechanism is provided on the shoulder of the seed germ bottle. The center of the circular opening receiving portion that receives the portion is arranged so as to be aligned with the axis of the rotation shaft of the rotary cutting portion, and the pressing mechanism that pushes out the bottom of the inoculum bottle centers the inoculum bottle. The rotary cutting portion has a cutting blade that slides back and forth from the rotary cutting portion main body, and is provided with a predetermined cutting angle with respect to the shoulder portion of the inoculum bottle. A configuration was adopted in which the shoulder portion was opened after being cut off by the cutting blade and then rotated. [Selection diagram] Fig. 1【課題】本発明は、全自動接種機にセットする種菌瓶の開口部を設ける装置において、低コストで、簡易に操作でき、正確な口切と無菌状態を維持可能な種菌瓶開口装置の提供を課題とするものである。【解決手段】種菌瓶の肩部を開口する開口装置であって、本体フレームに、芯出し機構と、押圧機構と、回転切断部とを備え、前記芯出し機構は、前記種菌瓶の前記肩部を受ける円状の開口受部の中心が前記回転切断部の回転軸の軸芯上に整合するように配置され、前記種菌瓶の底部を押し出しする前記押圧機構によって前記種菌瓶の芯出しを行い、前記回転切断部は、回転切断部本体から前後に摺動する切断刃を有するとともに、前記種菌瓶の前記肩部に対して所定の切り込み角を有して設けられ、前記種菌瓶に前記切断刃により欠切後、回転して前記肩部を開口する構成を採用した。【選択図】図1