Disclosed is a method for simply and efficiently separating a sialyllactose material starting with a milk material. Sialyllactose can be concentrated and separated by a simple method comprising deproteinizing a milk material, adjusting the pH value of the deproteinized solution thus obtained and concentrating the pH-adjusted solution using an ultrafiltration membrane (UF membrane). Since lactose and mineral fractions can be also separated by the aforesaid method, sialyllactose having a high purity can be obtained thereby. Therefore, the sialyllactose material thus obtained is highly valuable as foods, pharmaceuticals and so on.
