[Object] To obtain an anti-integrin α8β1 antibody which binds to integrin α8β1 derived from mammals of different species. Also, to obtain an anti-integrin α8β1 antibody which inhibits binding between integrin α8β1 and its ligand.[Solution] Anti-integrin α8β1 antibodies which bind to integrin α8β1 derived from mammals of different species have been obtained. In addition, inhibitors, which contain an anti-integrin α8β1 antibody, of binding between integrin α8β1 and its ligand have been obtained.