PURPOSE: A manufacturing method of Lentinus edodes culture material is provided to bottle a solid medium in a wide-mouth bottle, inject liquid fungus, culture the fungus, take the solid medium out of the bottle, put the medium into a bag, and culture the medium. CONSTITUTION: A manufacturing method of Lentinus edodes culture material comprises the following steps: (A) Manufacturing a solid fungus by mixing 100 wt% of sawdust and 10-40 wt% of rice bran and adding 150-250 wt% of water； (B) putting the solid fungus in a wide-mouth bottle, sterilizing and cooling the same at 100-135 deg.C. for 2-3 hours in a sterilizing room； (C) injecting 5-15 wt% of Lentinus edodes culture medium into the solid fungus based on 100 wt% of sawdust, and culturing the medium at 15-25 deg.C. for 40-55 days under a dark condition； and (D) taking the cultured material out of the wide-mouth bottle, putting the cultured material into a porous bag, and bottle-culturing the cultured material at 15-25 deg.C. for 45-60 days. The Lentinus edodes culture medium comprises: 100 wt% of water, 2-5 wt% of sugar, 0.2-0.5 wt% of yeast extract, 0.03-0.1 wt% of KH2PO4, 0.03-0.1 wt% of MgSO4.7H2O, and 0.05-0.2 wt% of KNO3. 10-20 wt% of wheat bran per 100 wt% of sawdust is additionally added to the solid fungus of step (A). 5-10 wt% of cotton seed hull per 100 wt% of sawdust is additionally added to the solid fungus of step (A). The humidity for putting the culture material in the porous bag in step (D) is 1-60%.본 발명은 표고버섯 배양물 제조방법에 관한 것이다. 구체적으로, 본 발명은 고체배지를 광구병에 입병하고 액체종균을 접종 및 배양한 후 상기 고체배지를 탈병하고 다시 봉지에 입봉하여 배양하는 것을 특징으로 하는 표고버섯 배양물 제조방법에 관한 것이다.
