This invention provides a matrix scaffold for three-dimensional (3D) cell cultivation, a method of its construction, and its uses. The matrix scaffold results from a crosslinking reaction of a fibroin-like substance prepared by a specific method from silk cocoons or filaments, chitosan and crosslinking agents. This matrix scaffold is more advantageous in terms of degradation stability and benefits to 3D cell cultivation over existing matrix scaffolds. In addition, it can replace extracellular matrices (ECMs) or tissue and organ matrices and is further for use for the in-vitro differentiation and proliferation of cells, tissue and organ reconstruction and antitumor drug screening.
