The present disclosure provides a culture method for improving amount of polysaccharides in inonotus obliquus, and the culture method includes the following steps. First, a strain of the inonotus obliquus is inoculate to a solid medium for producing mycelium of the inonotus obliquus. When the area of the cultivated mycelium of the inonotus obliquus is up to a first predetermined value, a brightness and a temperature of the environment, where the mycelium of the inonotus obliquus is cultured, are lowered until a nutrient solution is added to the solid medium. When the area of the cultivated mycelium of the inonotus obliquus is up to a second predetermined value, a harvesting step is performed. Therefore, the amount of the polysaccharides in the inonotus obliquus can be improved through the present disclosure.本發明提供一種用以提高白樺茸中多醣體含量之固態培養方法,其包含接種步驟、第一培養環境調整步驟、添加營養液步驟、第二培養環境調整步驟以及採收步驟。首先,將白樺茸菌株接種至固態培養基以培養出白樺茸菌絲體。當白樺茸菌絲體達第一預定面積時,降低培養環境之亮度與溫度後,並於避光低溫環境中培養一培養時間。接著,在添加營養液後,回復培養環境之亮度與溫度,並待白樺茸菌絲體達第二預定面積時採收。藉此,本發明可有效提升白樺茸菌絲體產物中三萜類化合物的含量。S100、S102、S104、S106、S108、S110‧‧‧步驟
