MISTRY, Sanjay,MESSINA, Darin,HARRIS, Ian,HARMON, Alexander,KIHM, Anthony,SEYDA, Agnieszka,YI, Chin-Feng,GOSIEWSKA, Anna
申请号：
ES04777234
公开号：
ES2600555T3
申请日:
2004.06.25
申请国别(地区):
ES
年份:
2017
代理人:
摘要：
Multipotent or pluripotent cells isolated from the human umbilical cord for use in the treatment of an ocular degenerative condition in a patient, where cells can be obtained from human umbilical cord tissue substantially free of blood and where the cells have the following characteristics: . potential to experience at least 40 duplications in culture； b. binding and expansion in a coated or uncoated tissue culture vessel, wherein the coated tissue culture vessel comprises a coating of gelatin, laminin, collagen, polyiorithin, vitronectin or fibronectin； C. production of vimentin and actin of smooth muscle alpha； d. production of each of CD10, CD13, CD44, CD73, HLA-A, B, C, PDGFr-alpha and CD90 as detected by flow cytometry； and. increased expression of endogenous genes encoding interleukin 8； reticulon 1； chemokine ligand 1 (motif C-X-C)； chemokine ligand 3 (motif C-X-C)； chemokine ligand 6 (C-XC motif) and protein 3 induced by tumor necrosis factor alpha in relation to endogenous expression of interleukin 8, reticulon 1, chemokine ligand 1 (motif C-X-C)； chemokine ligand 3 (motif C-X-C)； ligand 6 of chemokines (CXC motif) and protein 3 induced by tumor necrosis factor alpha in a human cell that is a fibroblast, a mesenchymal stem cell, or a bone marrow cell of the iliac crests, as characterized by matrix of oligonucleotides； F. lack of production of CD31, CD34, CD45, CD 117 and CD141 as detected by flow cytometry； g. they are able to self-renew and expand； h. growth in about 5% to about 20% oxygen； i. require L-valine for growth； j. they lack expression of HLA-DR, HLA-DP, HLA-DQ, CD80, CD86, B7-H2, HLA-G and CD 178 as detected by flow cytometry； k. PD-L2 expression as detected by flow cytometry； l. secretion of MCP-1, IL-6, GCP-2, IL-8, TIMP1, TPO, KGF, HGF, FGF, HBEGF and BDNF as detected by ELISA； m. lack of secretion of SDF-1 alpha, VEGF, TGF-beta2, ANG2 and PDGFbb as detected by ELISA； and n. a decrease in the expression of the following genes in relation to a
