MISTRY, Sanjay,KIHM, Anthony J.,HARRIS, Ian Ross,HARMON, Alexander M.,MESSINA, Darin J.,SEYDA, Agnieszka,YI, Chin-Feng,GOSIEWSKA, Anna
申请号：
ES04756395
公开号：
ES2564044T3
申请日:
2004.06.25
申请国别(地区):
ES
年份:
2016
代理人:
摘要：
An isolated cell obtainable from human umbilical cord tissue, wherein said human umbilical cord tissue is substantially free of blood, said cell is capable of self-renewal and expansion in culture and has the potential to differentiate into cells of other phenotypes, said cell is capable of expanding in the presence of oxygen from about 5% to about 20%, said cell requires L-valine for growth, and wherein said cell has the following characteristics: a. potential to experience at least 40 duplications in culture； b. binding and expansion on a coated or uncoated tissue culture vessel, wherein the coated tissue culture vessel comprises a coating of gelatin, laminin, collagen, polyiorithin, vitronectin or fibronectin； C. smooth muscle vimentin and alpha-actin production； d. production of cell surface markers CD10, CD13, CD44, CD73, HLA-A, B, C, CD90 and PDGFr-alpha, as detected by flow cytometry； and. high expression of endogenous genes encoding interleukin 8, reticulon 1, chemokine ligand 1 (motif C-X-C) (melanoma growth stimulating activity, alpha)； chemokine ligand 6 (CX-C motif) (granulocyte chemotactic protein 2)； chemokine ligand 3 (motif C-X-C)； protein 3 induced by tumor necrosis factor alpha, with respect to the endogenous expression of interleukin 8, reticulon 1, ligand 1 of chemokines (motif C-X-C) (melanoma growth stimulating activity, alpha)； chemokine ligand 6 (motif C-X-C) (granulocyte chemotactic protein 2)； chemokine ligand 3 (motif C-XC)； protein 3 induced by tumor necrosis factor alpha in a human cell that is a fibroblast, a mesenchymal cytoblast, or a bone marrow cell of the iliac crests, as characterized by oligonucleotide matrix； F. absence of production of CD31, CD34, CD45, CD117, CD141 and HLA-DR, DP, DQ, as detected by flow cytometry； g. secretion of MCP-1, IL-6, GCP-2, IL-8, TIMP1, TPO, KGF, HGF, FGF, HBEGF, BDNF and IL-8, as detected by ELISA； h. absence of secretion of SDF-1alpha, VEGF, TGF-beta2, ANG2 and PDGFbb, as detected by ELISA； i. abse
