A transfer system includes a first and a second processing region, a support base, an anesthetization unit and a cover handling unit. The support base can provide support for a plurality of culture containers in the first and second processing regions, each culture container including a tube having two openings and a removable cover installable to close one of the two openings of the tube, the first and second processing regions being adapted to interchangeably receive the tubes of the culture containers. The anesthetization unit is operable to deliver an anesthetic substance into at least one culture container positioned in the first processing region. The cover handling unit is operable to separate the covers from the tubes and close the openings of the tubes with the covers in the first and second processing regions. Moreover, embodiments described herein provide a method of transferring a cultured organism with the transfer system.