The present disclosure provides an eyeball locating method and system to track the eyes. The eyeball locating method and system is better than the present commercialized products. Tracking the eyes is an important evidence to detect whether the user is looking at the screen, and the eyeball locating method and system is widely used in electronic industrial. Therefore, The eyeball locating method and system has its potential market.本發明提供一種眼球定位方法及系統追蹤眼球,本發明眼球定位方法及系統,優於目前已知之眼球定位方法及系統。眼球定位方法及系統是偵測使用者是否有在看螢幕之依據,眼球定位方法及系統廣泛應用於民生與電子產業,潛在市場不容小覷。本發明之高速眼球定位方法及系統於電子商務產業具有明顯競爭優勢。100‧‧‧眼球定位方法110‧‧‧開啟視訊120‧‧‧設定關注區域130‧‧‧灰階化140‧‧‧濾波150‧‧‧二值化160‧‧‧虹膜中心估測164L‧‧‧左映射補償164R‧‧‧右映射補償170‧‧‧螢幕注視點估測