Disclosed in the present invention are a thawing fluid for cryopreservation, a preparation method therefor and a use thereof. The thawing fluid comprises: 0.1-50 g of a biomimetic material for controlling ice, 0-1.0 mol L -1 of a water-soluble sugar, and balance of a buffer solution, wherein the biomimetic material for controlling ice is a material for controlling ice having an ice-philic group and a hydrophilic group, the hydrophilic group is a functional group that can form a non-covalent interaction with water molecules, and the ice-philic group is a functional group that can form a non-covalent interaction with ice. In the present invention, by means of the thawing fluid and the thawing reagent prepared from the biomimetic material for controlling ice, which material has ice-philic and hydrophilic properties, the growth of ice crystals can be effectively controlled, and damage to cells or tissue caused by uncontrolled growth of the ice crystals during recovery can be significantly ameliorated. Moreover, the thawing fluid and the thawing reagent have a good biocompatibility, do not contain animal serum, and have a low toxicity. Compared with a traditional thawing fluid containing serum, the thawing fluid and the thawing reagent of the present invention reduce the risks of shelf life being short and introduction of parasitic biological contaminants, and are more conducive to maintaining the generation stability of cells or tissues. In addition, the present invention has simple components, is low in cost and has good application prospects.La présente invention concerne un liquide de décongélation, son procédé de préparation et son utilisation. Le liquide de décongélation comprend : 0,1 à 50 g d'une substance biomimétique pour lutter contre la glace, 0 à 1,0 mol L -1 d'un sucre hydrosoluble, et le reste étant une solution tampon, la substance biomimétique pour lutter contre la glace étant une substance pour lutter contre la glace ayant un groupe cryophile et un grou
