LIN, CHIA CHING,林佳静,林佳靜,HSIEH, CHO CHEN,谢卓臻,謝卓臻,CHANG, HSIN JU,张欣茹,張欣茹
申请号：
TW102133034
公开号：
TWI516596B
申请日:
2013.09.12
申请国别(地区):
TW
年份:
2016
代理人:
摘要：
The present invention is in relation to a kind of constituent and its preparation method for promoting cytothesis of what. What in vitro culture human body marrow mesenchymal stem cell first, human stem cells growth factor, white blood cell growth factor and a species of small clam living in fresh water extract are added in the DMEM last reign of a dynasty culture solution of serum-free, so that human bone marrow's mesenchymal stem cell can secrete a variety of human growth factors to form human growth factor's constituent, most Hou is embedded again with micro- rouge body embedding techniques of Water-In-Oil-oil-in-water (Water-in-Oil-in-Water, W/O/W). Constituent made of being embedded can promote epidermal tissue Shang Kou More to close regeneration, reduce scar generation, and can activate hair follicle melanocyte and hair follicle stimulating cytothesis via topical application mode. Furthermore also the regeneration maintenance of brain injury nigral cell and hippocampal gyrus area nerve cell can be promoted newborn via oral way, and stimulation marrow hemopoietic stem cells hyperplasia.本發明有關於一種促進細胞再生的組成物及其製備方式。首先於體外培養人體骨髓間葉幹細胞，在無血清的DMEM末代培養液中添加人類幹細胞生長因子、白血球生長因子及蜆萃取物，使得人體骨髓間葉幹細胞可分泌多種人類生長因子以形成人類生長因子組成物，最後再以油包水-水包油(Water-in-Oil-in-Water，W/O/W)的微脂體包埋技術包埋。經包埋而成的組成物可經由表皮塗抹方式，促進表皮組織傷口癒合再生、減少疤痕產生，並可活化毛囊黑色素細胞及刺激毛囊細胞再生。此外亦可經由口服方式，促進腦部受損黑質細胞再生修護與海馬迴區神經細胞新生，以及刺激骨髓造血幹細胞增生。
