The invention discloses a composition for promoting cell regeneration and a preparation method and use thereof. The composition for promoting cell regeneration provided by the invention is prepared by the following method: first, conducting in vitro culture of human bone marrow mesenchymal stem cells: adding a human stem cell growth factor, a granulocyte colony-stimulating factor and a clam extract, so that the human bone marrow mesenchymal stem cells can secrete a variety of human growth factors to form a human growth factor composition and finally embedding the composition by a Water-in-Oil-in-Water (W / O / W) liposome embedding technology. The composition obtained by embedding can smear on epidermis to promote e wound healing of epidermal tissue, reduce scar formation, and activate hair follicle melanocytes and stimulate hair cell regeneration. In addition, the composition can be taken orally to promote regeneration and repair of damaged brain substantia nigra cells and hippocampal gyrus neurogenesis, and stimulate the proliferation of bone marrow hematopoietic stem cells.
