A method for artificial cultivation of Ophiocordyceps sinensis fruiting bodies. The method comprises: inoculating Ophiocordyceps sinensis into a sterile rice medium, cultivating at 9-13° C. for 40-60 days, after the medium is covered with mycelia, performing low-temperature induction at 1-8° C. for 60-80 days to develop a fruiting body primordium, and transferring the cultivation to 11-16° C. till harvest of the fruiting bodies. The method provided by the present invention requires no low-oxygen environment, which can reduce cultivation cost it only needs 3-4 months from induction to harvest of fruiting bodies the rice medium for use has a low cost, which is suitable for commercial cultivation of Ophiocordyceps sinensis fruiting bodies.
