Provided is a biological observation apparatus including irradiating portions that radiate illumination light onto biological tissue, an imaging portion that, of reflected light reflected at the biological tissue due to the illumination light radiated by the irradiating portions, captures reflected light in a wavelength band in which an absorption characteristic of β-carotene is greater than an absorption characteristic of hemoglobin, thus acquiring a reflected-light image, and a display portion that displays the reflected-light image acquired by the imaging portion.