Disclosed is a fluorescence observation device (1) which is provided with: an illuminating section (3) which irradiates illuminating light and excitation light on a subject (A) a fluorescence image acquisition unit (16) which takes an image of the fluorescence generated in the subject (A) due to the irradiation of the excitation light, and acquires a fluorescence image a returned light image acquisition unit (17) which takes an image of the returned light returning from the subject (A) due to the irradiation of the illuminating light, and acquires a returned light image a light distribution properties information storage unit (21) which stores information relating to the light distribution properties of optical systems (14, 15) provided in the image acquisition units (16, 17) an image revision unit (21) which uses the information relating to the light distribution properties to revise at least the fluorescence image and/or the returned light image so as to harmonise the light distribution properties included in the fluorescence image and the returned light image and an image standardising unit (22) which uses the revised fluorescence image and returned light image to standardise the fluorescence image according to the returned light image.La présente invention concerne un dispositif dobservation de fluorescence (1) qui est pourvu de : une section dillumination (3) qui irradie une lumière dillumination et une lumière dexcitation sur un sujet (A) une unité dacquisition dimage de fluorescence (16) qui prend une image de la fluorescence générée dans le sujet (A) grâce à lirradiation de la lumière dexcitation, et obtient une image de fluorescence une unité dacquisition dimage de lumière retournée (17) qui prend une image de la lumière retournée retournant depuis le sujet (A) en raison de lirradiation de la lumière dillumination, et obtient une image de lumière retournée une unité de stockage dinformations de propriétés de distribution de lumière (21) qui stocke des in
