A position at a surface of a target organ or the like to be removed can be made easier to view, and nerves surrounding the target organ can be prevented from being damaged in advance. Provided is a biological observation apparatus 1 including irradiating portions 3 and 7 that radiate illumination light onto biological tissue, an imaging portion 8 that, of reflected light reflected at the biological tissue due to the illumination light radiated by the irradiating portions 3 and 7, captures reflected light in a wavelength band in which an absorption characteristic of β-carotene is greater than an absorption characteristic of hemoglobin, thus acquiring a reflected-light image, and a display portion 6 that displays the reflected-light image acquired by the imaging portion 8.
