The present invention relates to a cauliflower mushroom spawn infection- and air pollution-preventing mass inoculation system including: a medium preparation unit preparing a medium and transporting it in a first direction； sterilization and cooling units communicating with the medium preparation unit, sequentially sterilizing and cooling the medium supplied from the medium preparation unit, and transporting the medium in a second direction perpendicular to the first direction； an inoculation unit communicating with the cooling unit, inoculating the medium cooled by the cooling unit with a cauliflower mushroom spawn, and transporting the medium in a third direction perpendicular to the second direction； an air supply unit supplying purified air for a positive pressure to be maintained in the inoculation unit； and an air discharge unit forming an air discharge path through which the air in the inoculation unit is discharged to the outside. According to the cauliflower mushroom spawn infection- and air pollution-preventing mass inoculation system of the present invention, air flow is partially blocked by the direction in which the medium is transported being repeatedly and perpendicularly converted, and thus contaminant inflow into the inoculation unit can be minimized. Since the positive pressure is maintained in the inoculation unit, external contaminant inflow into the inoculation unit can be forestalled. Because a spawn cultivation section is placed to be surrounded by the medium preparation unit, the sterilization and cooling units, and the inoculation unit, the cultivated cauliflower mushroom spawn can be given a minimized line of movement and the spatial utilization efficiency of devices constituting the system can be improved.본 발명에 따르면, 배지를 준비하여 제1방향으로 이송시키는 배지준비부； 상기 배지준비부와 연통하여 상기 배지준비부로부터 공급된 배지를 순차적으로 살균 및 냉각시키는 것으로, 상기 배지를 상기 제1방향과 수직을 이루는 제2방향으로 이송시키는 살균 및 냉각부； 상기 냉각부와 연통하여 상기 냉각부에서 냉각된 배지에 꽃송이버섯 종균을 접종하는 것으로, 배지를 상기 제2방향과 수직을 이루는 제3방향으로 이송시키는 접종부； 상기 접종
