Federalnoe gosudarstvennoe byudzhetnoe uchrezhdenie nauki Institut biologii morya im. A.V. ZHirmunskogo Dalnevostochnogo otdeleniya Rossijskoj akademii nauk (IBM DVO RAN)
发明人:
Kumejko Vadim Vladimirovich,Кумейко Вадим Владимирович,SHCHeblykina Anna Vladimirovna,Щеблыкина Анна Владимировна,Dyujzen Inessa Valerevna,Дюйзен Инесса Валерьевна,KHotimchenko YUrij Stepanovich,Хотим
申请号：
RU2013147902/05
公开号：
RU0002597085C2
申请日:
2013.11.07
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention relates to medicine, particularly to a new matrix material for tissue bioengineering and regenerative medicine and methods of its production. Matrix material is designed based on vegetal polysaccharide - pectin with etherification degree of not more than 50% and proteins of intracellular matrix-collagens I and IV types, which contains said components in the following concentrations: 0.5-2.0 wt%, 0.1-1.5 wt% and 0.01-0.5 wt%, respectively. Method of production of the implanted matrix material for regenerative medicine in the form of hydrogel involves mixing at a temperature of 5 °C max of calcium acetate solution of type I collagen to final concentration of 0.1-1.5 wt% with type IV collagen solution to the final concentration 0.01-0.1 wt%, then the produced mixture is mixed with previously prepared gel formation initiator, including calcium chloride solution in the concentration, providing a gel forming process, non-toxic buffer system in amount sufficient for neutralisation of the mixture, and sodium chloride solution up to physiological concentration then the pectin solution is introduced with degree of esterification of 50% max to the final concentration 0.5-2.0 wt% and the gel is formed by temperature rise up to physiological. Invention also relates to a method of producing implanted matrix material for regenerative medicine in the form of hydrogel, which involves mixing at a temperature of 5 °C max of calcium acetate solution of type I collagen to final concentration of 0.1-1.5 wt% with IV type collagen solution to the final concentration 0.01-0.1 wt%, then the produced mixture is neutralised, stabilised by the non-toxic buffer system and the sodium chloride is added up to physiological concentration then the pectin solution with etherification degree of not more than 50 % to the final concentration 0.5-2.0 wt% is added, to obtain a fluid composition, from which the gel is formed.EFFECT: invention enables to obtain a biocompa
