Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Кубанский государственный аграрный университет" (RU)
发明人:
Ольховатов Егор Анатольевич (RU),Родионова Людмила Яковлевна (RU),Щербакова Елена Владимировна (RU)
申请号：
RU2011138126/13
公开号：
RU2011138126A
申请日:
2011.09.16
申请国别(地区):
RU
年份:
2013
代理人:
摘要：
FIELD: food industry.SUBSTANCE: invention relates to technical biochemistry, in particular - to determination of pectin substances quantity in vegetal raw material. "Standardised" solutions of pectin substances fractions are prepared. The fractions solutions are sampled. Saponification of pectin substances fractions in the samples of the solutions being analysed is performed with 2.5 ml of 40% NaOH solution. The pectin substances fractions are sedimented with 2.5 ml of concentrated HCl. The solutions being analysed are centrifuged in 50 ml test tubes, rotation frequency being no less than 500 rpm, during 5-7 minutes. The sediments of pectin substances fractions are suspended in distilled water in a titration cup on a magnetic stirrer for at least 30 minutes. One performs conductometric titration of the suspension of pectin substances fractions sediment, stirring. Following the conductometric titration results, graphs are drawn, relying whereon one identifies the volume of the titrant spent on pectin acid titration. Pectin substances fractions weight percentage is calculated from the formula: ω=176×0,2×V10×200×100, where 176 - pectin acid equivalent 0.2 - titrant normality V - titrant volume spent on pectin acid titration, ml 200 - volume of the solution of the corresponding pectin substances fraction taken for analysis, ml 10 - conversion to acid milliequivalents (1 ml of 0.1n NaOH corresponding to 0.1 acid milliequivalent) 100 - percentage conversion factor.EFFECT: invention enables obtainment of accurate data with high coincidence of parallel analyses and ensures labour and time expenditures saving at all the process stages.6 dwg, 1 exИзобретение относится к технической биохимии, а именно к определению количества пектиновых веществ в растительном сырье. Готовят «стандартизованные» растворы фракций пектиновых веществ. Отбирают пробы растворов фракций. Омыление фракций пектиновых веществ в пробах анализируемых растворов проводят 2,5 мл 40%-ного раствора NaOH. Фракц
