Compositions and methods are provided for therapeutic treatment using recombinant Hemopexin molecules having sufficient sialyation and/or absence of neutral glycans to allow for sufficient circulation to remove free heme from a biological organism. In other embodiments, a recombinant Hemopexin molecule is provided for therapeutic treatment having a percentage of neutral glycans to total glycans in a range of from about 2 to about 30 percent as measured by HPLC after labelling with fluorescent probe 2-aminobenzoic acid. Methods of treatment and making a recombinant Hemopexin molecule are also described.使用具有充分唾液酸化及/或缺乏中性聚糖之重組的凝血酶分子，使其得以充份循環以便將游離的血基質從生物體中移除，來提供用於治療性治療之組成物和方法。在其他的實施例中，係提供一用於治療性治療之重組的凝血酶分子，其具有中性聚糖佔總聚糖之百分比係在約百分之2至約百分之30的範圍，如經螢光素探針2-胺基苯甲酸標記後以HPLC所測。亦描述治療和製造重組凝血酶分子之方法。
