This invention relates to the development of a method and process of enrichment of a fructan of Agave Tequilana Weber and Oaxacan Agave Angustifolia Haw in a prebiotic drink with unique characteristics that offers the prebiotic effect in probiotic and animal strains, using the in vitro and in vivo method. The developed process of enrichment protects the unique fructan against the polimerization grade change, thus allowing the preservation of its innate prebiotic characteristics.