PURPOSE: A producing method of food using lactobacillus fermentum JS strains is provided to improve the texture and flavor of the food, and to offer the abundant content of lactobacillus to users. CONSTITUTION: A producing method of food using lactobacillus fermentum JS strains comprises the following steps: culturing the lactobacillus fermentum JS strains in a vegetable lactobacillus medium; inserting 0.1-1 part of lactobacillus fermentum JS strains by weight into 100 parts of grain by weight; and settling the mixture for 30 minutes-10 hours, and cooking the mixture at 90-110 deg C to obtain steamed cake. The vegetable lactobacillus medium contains 400g of glucose, 400g of bean extract powder, 50g of K2HPO4, 40g of sodium acetate, 10g of MgSO4, and 10g of Tween-80 for 20L of water. The grain is selected from rice, non-glutinous rice, glutinous rice, barley, millet, sorghum, oat, or bean.본 발명은 락토바실러스 퍼멘텀 JS 균주를 식물성유산균 배지에서 배양하는 단계; 및 배양된 균주를 곡류에 첨가하여 밥, 떡, 도넛을 제조하는 단계를 포함하는 락토바실러스 퍼멘텀 JS 균주를 이용한 식품의 제조방법에 관한 것이다. 본 발명에 따르면, 질감과 풍미가 좋고 유산균이 풍부할 뿐 아니라, 노화방지 및 저장성이 우수한 식품이 제조될 수 있다.