KALE SAMEER MANOHAR,SHARMA PANKAJ KESHAV,Gairola Sunil Jagdishprasad
申请号：
IN2414/MUM/2014
公开号：
IN2014MU02414A
申请日:
2014.07.25
申请国别(地区):
IN
年份:
2016
代理人:
摘要：
The present invention relates to the field of immunoassays for Gram negative bacteria, in particular N. meningitidis. The invention provides a simple and affordable immunoassay to quantitate polysaccharides in meningococcal vaccines for the evaluation of antigen content and lot-to-lot manufacturing consistency. The inventors have found a Sandwich ELISA that can be applicable for the quantitation and identification of N.meningitidis serogroup X polysaccharide in a multivalent meningococcal polysaccharide-protein conjugate vaccine as well as in a multivalent meningococcal plain polysaccharide vaccine.Said assay employs a polyclonal antibody as capture antibody and a novel monoclonal antibody against serogroup X polysaccharide as detection antibody.Further the assay is rapid, robust and reproducible.
