PROCESS FOR PRODUCTION AND PURIFICATION OF CAPSULAR POLYSACCHARIDE FROM HAEMOPHILUS INFLUENZA TYPE B AND METHOD FOR PRODUCING A CONJUGATE VACCINE COMPOSITION USING SAID POLYSACCHARIDE.
The present invention relates to the production and purification of Capsular polysaccharide from Haemophilus influenza type b and more particularly to the method for producing a conjugate vaccine composition using said polysaccharide. Therefore the object of the present invention is to provide a process for the production and purification of Polyribosylribitol Phosphate (PRP) from Haemophilus influenza by treatment of seed inoculum and using it for large scale high yielding production of polysaccharide from Haemophilus influenza type b. Yet another object of the present invention is to provide a method for conjugation of Polyribosylribitol Phosphate (PRP) with carrier protein such as Tetanus Toxoid, Diphtheria toxoid, CRM protein etc for preparation of conjugate vaccine.
