IMPROVED PROCESS FOR PRODUCTION AND PURIFICATION OF CAPSULAR PNEUMOCOCCAL POLYSACCHARIDE FROM STREPTOCOCCUS PNEUMONIAE AND METHOD FOR PRODUCING CONJUGATE VACCINE COMPOSITION USING SAID POLYSACCHARIDE
The present disclosure provides a culture medium capable of being used for clonal growth of Streptococcus pneumoniae to produce capsular pneumococcal polysaccharide for vaccine production, wherein the culture medium can include KH2PO4, NaHCO3, soya peptone, yeast extract, MgSO4, NaCl, CaCl2, glucose and L-cysteine or its salts. The present disclosure further provides a method of fermentation of Streptococcus pneumoniae to produce capsular pneumococcal polysaccharide.