A method of preparing an extract from Peony’s root cortex is disclosed, which is performed by at least one extraction step and a gel chromatography step, so as to obtaining the extract from Peony’s root cortex. In addition, the extract from Peony’s root cortex obtained by the aforementioned method is disclosed, in which the extract from Peony’s root cortex includes the relatively high amount of total phenolic compounds and monoterpene glycosides, for example, tetragalloyl glucose, pentagalloyl glucose, galloylpaeoniflorin, benzoyl paeoniflorin and mudanpioside B, so as to effectively provide an antioxidative activity for preventing or delaying skin aging, thereby being applied to the cosmetic composition and food additives with antioxidative activity.本發明揭露一種牡丹皮萃取物之製造方法,其係利用至少一萃取步驟以及凝膠層析步驟,以獲得牡丹皮萃取物。此外,本發明更揭露一種根據前述方法獲得之牡丹皮萃取物,其中此牡丹皮萃取物含有高量的總多酚化合物以及單萜配醣體類化合物,例如四沒食子醯基葡萄糖、五沒食子醯基葡萄糖、沒食子醯基芍藥苷、苯甲醯基芍藥苷以及牡丹皮苷B。因此,此牡丹皮萃取物具有抗氧化活性,有效避免或延緩皮膚老化的現象,進而可應用於抗氧化之化妝品組合物及/或食品添加物。101...牡丹皮材料103...第一萃取物105...第二萃取物107...第三萃取物109...第四萃取物111...第五萃取物113...牡丹皮萃取物
