A method of preparing an extract from Peony’s root cortex is disclosed, which is performed by at least one extraction step and a gel chromatography step, so as to obtaining the extract from Peony’s root cortex. In addition, the extract from Peony’s root cortex obtained by the aforementioned method is disclosed, in which the extract from Peony’s root cortex includes mudanpioside E, mudanpioside B and suffruticoside E, so as to effectively provide an anti-melanogenic activity, to prevent or to delay pigmentation, thereby being applied to the cosmetic composition and food additives for skin whiteness.本發明揭露一種牡丹皮萃取物之製造方法,其係利用至少一萃取步驟以及凝膠層析步驟,以獲得牡丹皮萃取物。此外,本發明更揭露一種根據前述方法獲得之牡丹皮萃取物,其中此牡丹皮萃取物可包括牡丹皮苷E、牡丹皮苷B以及牡丹酚E。此牡丹皮萃取物可提供抗黑色素生成的能力,有效避免或延緩色素沉澱的現象,進而可應用於皮膚美白之化妝品組合物及/或食品添加物。101...牡丹皮材料103...第一萃取物105...第二萃取物107...第三萃取物109...第四萃取物111...第五萃取物113...牡丹皮萃取物
