PROBLEM TO BE SOLVED: To provide microalgae with high production volume of lipid on inorganic salt medium, and a manufacturing method of lipid using the microalgae.SOLUTION: Production volume of lipid when cultured in an inorganic salt medium under the condition of pH 6.8, temperature at 25°C, illuminance on medium surface at 6000 lux is 50 mg/L/day or more, Oogamochlamys zimbabwiensis symbiotic with genus Acidovorax bacteria is cultured, lipid is extracted from the cultured Oogamochlamys zimbabwiensis symbiotic with genus Acidovorax bacteria. It is possible to manufacture lipid derived from microalgae at low cost by the manufacturing method of lipid. Further, since the manufacture lipid contains much unsaturated fatty acid, it is useful as lipid which has low freezing point and is usable in cold climate.