A dry stable probiotic composition is provided. The composition comprises one or more viable probiotic microorganisms, one or more hydrolyzed proteins, one or more disaccharides, one or more oligosaccharides, and one or more polysaccharides, but not trehalose. The composition has viability of at least 1x1010CFU/g, and a viability loss of less than 1 log unit/g after 3 months at a temperature of 40 °C and a relative humidity of 33%. Also provided are methods for preparing the dry stable probiotic composition.本發明提供乾穩定益生菌組合物。該組合物包含一或多種活益生菌微生物、一或多種水解蛋白質、一或多種二醣、一或多種寡醣及一或多種多醣，但不包含海藻糖。該組合物在40℃之溫度及33%之相對濕度下3個月後具有至少1×1010CFU/g之活力及小於1 log單位/g之活力損失。亦提供製備該乾穩定益生菌組合物之方法。
