A ferment method of lemon leaf for improving DPPH free radial scavenger activity is disclosed. The ferment method comprises: providing a lemon leaf providing a yeast mix the lemon leaf and the yeast to obtain a mixture with the yeast that final concentration is 5×107~1×108 CFU/ml and ferment the lemon leaf for 1~5 day to obtain a fermented the lemon leaf. Due to the fermented lemon leaf can obtain the lemon leaf of high DPPH free radial scavenger activity. As such botain a grocery which made by the lemon leaf with high DPPH free radial scavenger activity that can make consumer in good health.一種提升清除DPPH自由基活性的檸檬葉發酵方法,包含:提供一檸檬葉;提供一酵母菌菌株之菌液;混合該檸檬葉及該酵母菌菌株之菌液,以形成一混合物,使該酵母菌菌株於該混合物中之最終濃度為5×107~1×108CFU/ml;及於17~37℃之溫度下,以該酵母菌菌株發酵該檸檬葉1~5天,以獲得一發酵檸檬葉。藉由以該酵母菌菌株發酵該檸檬葉,以獲得高清除DPPH自由基活性的該發酵檸檬葉,達到使用該發酵檸檬葉所製作的食品具有高抗氧化活性以供食用者養生的功效。