PROBLEM TO BE SOLVED: To provide a method for efficiently producing naturally-derived isovaleric acid having no unusual odor like chemicals and suitable for food application.SOLUTION: A production method of a culture containing isovaleric acid includes a process for culturing an yeast that belongs to Pichia and has capacity to produce isovaleric acids in a medium that contains 20-50 mM of leucine.COPYRIGHT: (C)2014,JPO&INPIT【課題】薬品的な異風味がなく食品利用に適した天然物由来のイソ吉草酸を効率良く製造する方法の提供。【解決手段】ピキア属に属し、且つイソ吉草酸を生産する能力を有する酵母を、20~50mMのロイシンを含有する培地中に培養する工程を含む、イソ吉草酸を含む培養物の製造方法。【選択図】なし